The Master’s in Applications of Spatial Information (MAIE), jointly offered by CONAE and the National University of Córdoba through the Gulich Institute, represents a pioneering initiative in Latin America for the training of professionals in applied Earth observation. Over 16 years and with more than 100 graduates, the program has built a strong interdisciplinary foundation that integrates space technology with problem-solving approaches relevant to the Global South. This study presents a comprehensive analysis of the program’s evolution, including student demographics, funding mechanisms, and thesis topics. It highlights the program’s unique strengths, such as its alignment with the National Space Plan, personalized research mentorship, and partnerships with institutions like the Italian Space Agency. Thesis research spans diverse themes—ranging from environmental and disasters monitoring, epidemiology to food security and geospatial data science—employing varied satellite data sources. The findings underscore MAIE’s critical role in fostering technological sovereignty, sustainable development, and territorial intelligence, while also identifying areas for improvement in terms of equity and financial sustainability. In doing so, the paper positions MAIE as a key knowledge infrastructure for addressing socio-environmental challenges in Latin America and beyond.