As dynamic accessibility depends on the interplay of opportunities and travel impedance, available General Transit Feed Specification data and mobile phone data offer new opportunities to enhance the temporal sensitivity of accessibility. This paper utilizes a mobile phone-based origin-destination (OD) matrix and dynamic travel time via public transport to explore spatial and temporal patterns of accessibility measures. Four scenarios are constructed and compared to illustrate the relative accessibility. The results show that the impact of the public transport system changes progressively depending on the cut-off time considered, and average accessibility gains could vary four-fold between the city centre and suburban zones. Besides, the exploration of the relationship between land use, mobile phone-based attractiveness, and dynamic accessibility underlines the time-dependent effect of land use. Further research should focus on the development of advanced accessibility-based travel models.